Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium

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Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without MagnesiumMagnesium sulphate, MgSO4. 7H2O is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of magnesium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 2 gram powdered medium in 1 litre distilled water Store

0 % Store dry at room temperature

HYGROSCOPIC Store at Room Temperature

The medium is optimized for isolating lactic Streptococci

Tryptone 12 Yeast Extract 24 KH2PO4 2

01 Suspend 26

15 Suspend 55

LB-Agar High Salt

glycerol is not fermented to acetic acid

5 X sterile stock solution

30 g/L RO Water 695

Adjust to pH 6

MOPS (3-(N-morpholino)propanesulfonic acid